Selective isolation and distribution of Actinobispora strains in soil

Shin-ichi Suzuki*, Toru Okuda, and Saburo Komatsubara*  *Tanabe Seiyaku Co., Ltd., Discovery Research Laboratory

Can. J. Microbiol. 46: 708-715, 2000

A simplified enrichment method for selective isolation of Acinobisopra strains from soil is described.  Actinobispora spores were tolerant to dry-heat treatment at 110C for 15 min.  Actinobispora was more resistant to 1 mg/ml leucomycin, 1 mg/ml novobiocin, and 0.5 mg/ml tunicamycin than Streptomyces dominant in soil, which prevents selective isolation of Actinobispora.  Percentages of Actinobispora colonies on the isolation plate were increased by addition of antibiotics and dry-heat treatment of the soil samples.  By combining the techniques described above, this genus was isolated from 105 out of 574 soil samples (18% of the samples tested).  It was recovered from the soil samples with pH values ranging 5.0 to 8.9, and 78% of strains were isolated from neutral soil (pH 6.0-8.0).  A number of Actinobispora strains were isolated from various soils around the world.  Actinobispora strains are widely distributed in the world at relatively high frequency.

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